1JNH values show that N1...N3 hydrogen bonds are stronger in dsRNA A:U than dsDNA A:T base pairs |
| |
Authors: | Manalo Marlon N Kong Xiangming LiWang Andy |
| |
Institution: | Department of Biochemistry & Biophysics, Texas A&M University, College Station, Texas 77843-2128, USA. |
| |
Abstract: | Here, we show that 1JNH values are on average 0.4 Hz less negative for double-stranded RNA A:U than for DNA A:T base pairs, which, according to existing theory, suggests that RNA N1...N3 hydrogen bond distances are about 0.02 A shorter than those of DNA. Also, there is a statistically relevant trend between 1JNH and 2hDelta13C2 values, which supports the original hypothesis that 2hDelta13C2 values are also sensitive to hydrogen bond distances. Finally, a context dependence is observed for these values, which suggests that hydrogen-bonding and base-stacking interactions are coupled. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|