| 光谱法研究甲芬那酸与蛋白质的相互作用 |
| |
| 引用本文： | 潘祖亭,余军平.光谱法研究甲芬那酸与蛋白质的相互作用[J].分析试验室,2004,23(6):41-44. |
| |
| 作者姓名： | 潘祖亭 余军平 |
| |
| 作者单位： | 武汉大学化学与分子科学学院,武汉,430072;武汉大学化学与分子科学学院,武汉,430072 |
| |
| 基金项目： | 国家自然科学基金资助项目(20275028) |
| |
| 摘 要： | 用UV Vis吸收光谱和荧光光谱法研究了在模拟人体生理条件下,甲芬那酸与牛血清白蛋白结合反应特征,发现甲芬那酸对牛血清白蛋白有较强的荧光猝灭作用,且甲芬那酸的紫外吸收光谱和牛血清白蛋白的荧光发射光谱有一定程度的重叠,由此得出了其结合反应的结合常数、结合位点数和结合过程的基本热力学参数。
|
| 关 键 词： | 甲芬那酸 牛血清白蛋白 荧光光谱法 吸收光谱法 |
| 文章编号： | 1000-0720(2004)06-0041-04 |
Spectrometric study on the interaction between mefenamic acid and bovine serum albumin |
| |
| PAN Zu-ting and YU Jun-ping.Spectrometric study on the interaction between mefenamic acid and bovine serum albumin[J].Chinese Journal of Analysis Laboratory,2004,23(6):41-44. |
| |
| Authors: | PAN Zu-ting and YU Jun-ping |
| |
| Abstract: | The binding characteristics of mefenamic acid(MEA) and bovin serum albumin were studied by fluerescence and absorption spectroscopy in aqueous solution. The results showed that MEA had a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism. The fluorescence quenching data were analyzed according to Scatchard equation and double-reciprocal equation, and the binding constant, and the thermodynamic parameters et al. were obtained. |
| |
| Keywords: | Mefenamic Acid Bovine Serum Albumin Fluorescence Spectrum Absorption Spectrum |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
