| 反相高效液相色谱法同时测定姜黄药材中3个组分的含量 |
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| 引用本文： | 刘硕谦,刘仲华,黄建安,田娜,陆英,罗国安.反相高效液相色谱法同时测定姜黄药材中3个组分的含量[J].分析化学,2005,33(3):309-312. |
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| 作者姓名： | 刘硕谦 刘仲华 黄建安 田娜 陆英 罗国安 |
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| 作者单位： | 1. 湖南农业大学湖南省天然产物工程中心,长沙,410128
2. 清华大学化学系,北京,100084 |
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| 摘 要： | 建立了一种反相高效液相色谱方法,同时测定中药材姜黄块根中3组分的含量。采用KromasilTMC18柱(5μm,200mm×4.6mm),以磷酸水溶液(pH=2.5)和乙腈溶剂为流动相,梯度洗脱,流速0.9mL/min,柱温40℃,检测波长为425nm,10min内即可将3种组分分离测定。该方法快速、简便、准确,可为评价不同产地的姜黄质量提供依据。
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| 关 键 词： | 反相高效液相色谱 姜黄 姜黄素类化合物 |
Simultaneous Determination of Three Main Components in Curcuma longa L. By Reversed-phase High Performance Liquid Chromatography |
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| Liu Shuoqian,Liu Zhonghua,Huang Jianan,Tian Na,Lu Ying,Luo Guoan.Simultaneous Determination of Three Main Components in Curcuma longa L. By Reversed-phase High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,2005,33(3):309-312. |
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| Authors: | Liu Shuoqian Liu Zhonghua Huang Jianan Tian Na Lu Ying Luo Guoan |
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| Abstract: | A reversed-phase high performance liquid chromatographic method was established for simultaneous determination of curcumin, demethoxycurcumin and bidemethoxycnrcumin in the rhizome of Curcuma longa L., a typical herbaceous plant. A Kromasil~TM C_~18 column was used with a mixture of acetonitrile and phosphoric acid aqueous solution (pH=2.5) as the mobile phase. The flow rate was 0.9 mL/min and the detection wavelength was set at 425 nm. The three constituents were separated within 10 min. The method is rapid, simple and ~accurate , and it can be used for the evaluation of Curcuma longa L. |
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| Keywords: | Reversed-phase high performance liquid chromatography Curcuma longa L curcuminoids |
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