Amperometric l-lactate biosensor based on screen-printed carbon electrode containing cobalt phthalocyanine,coated with lactate oxidase-mesoporous silica conjugate layer |
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Authors: | Takeshi Shimomura Touru Sumiya Masatoshi Ono Tetsuji Ito Taka-aki Hanaoka |
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Institution: | 1. Funai Electric Advanced Applied Technology Research Institute Inc. (FEAT), TCI 37A, 2-1-6, Sengen, Tsukuba-shi, Ibaraki 305-0047, Japan;2. National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, Miyagino-ku, Sendai-shi, Miyagi 983-8551, Japan |
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Abstract: | A novel amperometric biosensor for the measurement of l-lactate has been developed. The device comprises a screen-printed carbon electrode containing cobalt phthalocyanine (CoPC-SPCE), coated with lactate oxidase (LOD) that is immobilized in mesoporous silica (FSM8.0) using a polymer matrix of denatured polyvinyl alcohol; a Nafion layer on the electrode surface acts as a barrier to interferents. The sampling unit attached to the SPCE requires only a small sample volume of 100 μL for each measurement. The measurement of l-lactate is based on the signal produced by hydrogen peroxide, the product of the enzymatic reaction. The behavior of the biosensor, LOD-FSM8.0/Naf/CoPC-SPCE, was examined in terms of pH, applied potential, sensitivity and operational range, selectivity, and storage stability. The sensor showed an optimum response at a pH of 7.4 and an applied potential of +450 mV. The determination range and the response time for l-lactate were 18.3 μM to 1.5 mM and approximately 90 s, respectively. In addition, the sensor exhibited high selectivity for l-lactate and was quite stable in storage, showing no noticeable change in its initial response after being stored for over 9 months. These results indicate that our method provides a simple, cost-effective, high-performance biosensor for l-lactate. |
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Keywords: | Screen-printed carbon electrode Cobalt phthalocyanine Lactate oxidase Mesoporous silica Nafion Sampling unit |
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