Determination of SCFAs in water using GC-FID. Selection of the separation system |
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Authors: | Anna Banel Anna JakimskaMarta Wasielewska Lidia WolskaBogdan Zygmunt |
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Affiliation: | Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology (GUT), 11/12 G. Narutowicza Street, 80-233 Gdańsk, Poland |
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Abstract: | In the present study, an analytical procedure was developed for the determination of short-chain fatty acids (SCFAs) in landfill leachate and municipal wastewater employing injection of aqueous samples to gas chromatograph with flame ionization detector (GC-FID). Chromatographic conditions such as a separation system, injection volume, oven temperature program were investigated and selected. With two columns, one with a polar (polyethylene glycol) and one with a non-polar (dimethylpolisiloxane) stationary phase, good separation of SCFAs, containing from 2 to 8 carbon atoms, was achieved. The sample volume was 2 μL and the temperature program 80 °C (30 s) then 7 °C min−1 to 220 °C (2 min). LOQs values were below 0.25 mg L−1. The concentrations of the acids in the landfill leachate studied ranged from 0.45 ± 0,059 (average ± extended uncertainty) mg L−1 for pentanoic acid to 15.2 ± 0.73 mg L−1 for ethanoic acid. Concentrations of SCFAs in the municipal wastewater were lower than LOQs. |
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Keywords: | Short-chain fatty acids (SCFAs) Chromatographic columns Gas chromatography Wastewater Landfill leachate |
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