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Three‐Dimensional Control of DNA Hybridization by Orthogonal Two‐Color Two‐Photon Uncaging |
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| Authors: | Manuela A. H. Fichte Xenia M. M. Weyel Dr. Stephan Junek Dr. Florian Schäfer Dr. Cyril Herbivo Prof. Dr. Maurice Goeldner Dr. Alexandre Specht Prof. Dr. Josef Wachtveitl Prof. Dr. Alexander Heckel |
| Affiliation: | 1. Institute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Frankfurt am Main, Germany;2. Imaging Facility, Max Planck Institute for Brain Research, Frankfurt am Main, Germany;3. Centre National de la Recherche Scientifique, Université de Strasbourg, Unité Mixte de Recherche 7199, Laboratoire de Conception et Application de Molécules Bioactives, équipe de Chimie et Neurobiologie Moléculaire, Illkirch, France;4. Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany |
| Abstract: | We successfully introduced two‐photon‐sensitive photolabile groups ([7‐(diethylamino)coumarin‐4‐yl]methyl and p‐dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three‐dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double‐strand displacement in a hydrogel and in neurons. Orthogonal two‐photon uncaging of the two cages is possible, thus enabling complex scenarios of three‐dimensional control of hybridization with light. |
| Keywords: | DNA hydrogels photochemistry two-photon uncaging wavelength selectivity |