Immobilization of the Gas Signaling Molecule H2S by Radioisotopes: Detection,Quantification, and In Vivo Imaging |
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| Authors: | Woonghee Lee Min Hwan Kim Phuong Tu Huynh Heesu Ahn Dr. Nikunj Bhatt Dr. Yong Jin Lee Dr. Jung Young Kim Prof. Kwon Moo Park Prof. Isao Ishii Prof. Shin‐Geol Kang Prof. Jeongsoo Yoo |
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| Affiliation: | 1. Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, Korea;2. Molecular Imaging Research Centre, Korea Institute of Radiological and Medical Sciences, Seoul, Korea;3. Department of Biochemistry, Keio University, Tokyo, Japan;4. Department of Chemistry, Daegu University, Gyeongsan, Korea |
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| Abstract: | Hydrogen sulfide (H2S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2S in live animals is very challenging. Herein, we report the first radioisotope‐based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2S. Macrocyclic 64Cu complexes that instantly reacted with gaseous H2S to form insoluble 64CuS in a highly sensitive and selective manner were prepared. The H2S concentration in biological samples was measured by a thin‐layer radiochromatography method. When 64Cu–cyclen was injected into mice, an elevated H2S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2S levels in a millimeter‐sized infarcted lesion of the rat heart. |
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| Keywords: | gas immobilization hydrogen sulfide imaging agents nuclear imaging radioisotopes |
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