Siloxane photopolymer to replace polydimethylsiloxane in microfluidic devices for polymerase chain reaction |
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Authors: | Alessandra Vitale Marzia Quaglio Stefano Turri Matteo Cocuzza Roberta Bongiovanni |
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Institution: | 1. Department of Applied Science and Technology, Politecnico di Torino, , 10129 Torino, Italy;2. Center for Space Human Robotics@PoliTo, Istituto Italiano di Tecnologia, , 10129 Torino, Italy;3. Department of Chemistry, Materials, and Chemical Engineering, Politecnico di Milano, , 20133 Milano, Italy;4. CNR‐IMEM, , 43124 Parma, Italy;5. Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali, , 50121 Firenze, Italy |
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Abstract: | Herein, we describe the preparation and characterization of a material suitable for the fabrication of microfluidic devices. The material is a silicone acrylic polymer, obtained by photopolymerization. It is polymerase chain reaction (PCR) compatible, resistant to temperature, optically transparent, and dimensionally stable; it has a better water and solvent resistance if compared with polydimethylsiloxane. Production of microfluidic layouts is successfully tested: a simple photolithographic approach allows to accurately control the pattern transfer and to produce PCR compatible microfluidic devices. The polymer characterization suggests that the proposed material satisfies all the characteristics required for an ideal PCR chip, without further treatment. Moreover, the possibility for fast, accurate, and cheap reproducibility of microdevices by liquid phase photopolymerization increases the polymer attractiveness. The material is a good alternative with respect to polydimethylsiloxane for the fabrication of microfluidic chips for biological analysis purposes. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | siloxane photopolymer PCR compatibility photolithography pattern transfer |
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