| 肝靶向海藻酸钠载药纳米粒的细胞毒性研究 |
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| 引用本文： | 张闯年,王蔚,王春红,田秦,黄微,袁直,陈学思.肝靶向海藻酸钠载药纳米粒的细胞毒性研究[J].中国科学B辑,2009,39(9):943-949. |
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| 作者姓名： | 张闯年 王蔚 王春红 田秦 黄微 袁直 陈学思 |
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| 作者单位： | 功能高分子材料教育部重点实验室;南开大学高分子化学研究所;高分子物理与化学国家重点实验室;中国科学院长春应用化学研究所; |
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| 基金项目： | 国家自然科学基金(批准号:20634030);;天津市自然科学基金(批准号:07JCZDJC00700);;中国科学院长春应用化学研究所高分子物理与化学国家重点实验室科学基金资助 |
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| 摘 要： | 本研究将具有肝靶向性分子甘草次酸（GA）偶联在具有生物相容性和生物可降解性的天然高分子海藻酸钠（ALG）上，合成了甘草次酸改性的海藻酸钠（GA—ALG）；对广谱抗癌药物阿霉素（DOX）进行包封，制备了肝靶向载药纳米粒，并考察了GA—ALG载药纳米粒的体外释药性能和对肝癌细胞的抑制作用．利用核磁、红外和元素分析技术对GA—ALG结构和GA取代度进行了表征；对GA—ALG载药纳米粒的形貌、粒径、表面Zeta电位等进行了测定，结果显示纳米粒具有较规则球形结构，其水合粒径为（214±11）nm．GA—ALG载药纳米粒在模拟生理条件下（pH7．4）可持续释药长达20天；MTT结果显示GA-ALG载药纳米粒对7703肝癌细胞的具有明显的杀伤作用．
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| 关 键 词： | 肝靶向 甘草次酸 海藻酸钠 细胞毒性 |
| 收稿时间： | 2009-05-17 |
| 修稿时间： | 2009-05-31 |
Study on cytotoxicity of liver targeted drug-loaded alginate nanoparticles |
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| ZHANG ChuangNian,WANG Wei,WANG ChunHong,TIAN Qin,HUANG Wei,YUAN Zhi & CHEN XueSi Key Laboratory of Functional Polymer Materials,Ministry of Education.Study on cytotoxicity of liver targeted drug-loaded alginate nanoparticles[J].Science in China(Series B),2009,39(9):943-949. |
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| Authors: | ZHANG ChuangNian WANG Wei WANG ChunHong TIAN Qin HUANG Wei YUAN Zhi & CHEN XueSi Key Laboratory of Functional Polymer Materials Ministry of Education |
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| Institution: | ZHANG ChuangNian1,WANG Wei1,WANG ChunHong1,TIAN Qin1,HUANG Wei1,YUAN Zhi1 & CHEN XueSi2 1 Key Laboratory of Functional Polymer Materials,Ministry of Education,Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China,2 State Key Laboratory of Polymer Physics , Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sci-ences,Changchun 130022 |
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| Abstract: | In this study,novel liver targeted doxorubicin(DOX) loaded alginate(ALG) nanoparticles were prepared by CaCl2 crosslinking method.Glycyrrhetinic acid(GA,a liver targeted molecule) modified alginate(GA-ALG) was synthesized in a heterogeneous system,and the structure of GA-ALG and the substitution degree of GA were analyzed by 1H NMR,FT-IR and elemental analysis.The drug release profile under the simulated physiological condition and cytotoxicity experiments of drug-loaded GA-ALG nanoparticles were carried ou... |
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| Keywords: | liver targeted glycyrrhetinic acid alginate cytotoxicity |
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