Nucleic acid and protein structures and interactions in viruses investigated by laser Raman spectroscopy |
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| Institution: | 1. Department of Analytical Chemistry, University of Chemistry and Technology, Technická 5, Prague 6 166 28, Czech Republic;2. Forensic Laboratory of Biologically Active Substances, Department of Chemistry of Natural Compounds, University of Chemistry and Technology, Technická 5, Prague 6 166 28, Czech Republic;3. Psychedelic Research Centre, National Institute of Mental Health, Topolová 748, Klecany 250 67, Czech Republic;4. Department of Solid State Chemistry, University of Chemistry and Technology, Technická 5, Prague 6 166 28, Czech Republic;1. Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-38 Krakow, Poland;2. Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland;3. Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland |
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| Abstract: | Raman spectroscopy may be profitably exploited to determine details of protein and nucleic acid structures and their mutual interactions in viruses and gene regulatory complexes. Present applications use data obtained from model nucleic acid crystals, fibers and solutions to reveal preferred backbone and nucleoside conformations for different morphological states of DNA and RNA in plant (TMV, BDMV) and bacterial viruses (P22, Pfl, Xf, Pf3, fd, Ifl, IKe). Interpretation of the results is enhanced by deconvolution methods which, in favorable cases, permit quantitative conclusions regarding macromolecular structures. Both equilibrium and dynamic Raman applications are described. |
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