直链淀粉手性固定相法分离利阿唑对映体

建立了利阿唑对映体的高效液相色谱拆分方法。采用Chiralpak AD-H手性柱在正相条件下直接拆分利阿唑对映体,考察了流动相中有机极性调节剂的种类和浓度、酸碱的种类和含量、柱温及流速等对利阿唑对映体分离的影响。确定了最佳的拆分条件:流动相为正己烷-乙醇(含0.3%二乙胺和0.1%冰醋酸)(体积比为80∶20),流速0.6 mL/min;检测波长254 nm;柱温20 ℃。在此条件下利阿唑对映体的分离度为3.4。该法简单快速,重现性好。

Enantiomeric separation of liarozole on amylose chiral stationary phase

A high performance liquid chromatographic method was established for the enantiomeric separation of liarozole. Baseline chiral separation of liarozole was achieved under normal-phase chromatographic mode by the Chiralpak AD-H chiral stationary phase. The influences of the concentration and kind of organic solvent, the proportion of acid and base, column temperature and flow rate on the enantiomeric separation were studied. The optimized chromatographic conditions were hexane-ethanol (containing 0.3% diethylamine and 0.1% glacial acetic acid) (80∶20, v/v) as the mobile phase with a flow rate of 0.6 mL/min and detection at the wavelength of 254 nm. The column temperature was set at 20 ℃. The resolution of 3.4 for liarozole was achieved under the above chromatographic conditions. The method is simple, rapid and with good reproducibility.