Effect of solvent composition on free release static coating of capillary columns |
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| Authors: | B. Xu N. P. E. Vermeulen J. A. M. Smit |
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| Affiliation: | (1) Department of Pharmacochemistry (Molecular Toxicology), Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands;(2) Centre for Bio-Pharmaceutical Sciences, State University Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands;(3) Department of Physical and Macromolecular Chemistry, Gorlaeus Laboratories, State University Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands |
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| Abstract: | Summary Three aspects with respect to the selection of solvents for static coating of capillary columns, i.e. coating speed, occurrence of bumping and solubility of stationary phases are discussed. Hypotheses are proposed in an attempt to explain the observed facts that mixed solvents result in much higher coating speeds than those obtained from pure solvents, that a proper choice of solvents together with a good and uniform deactivitation of the column inner wall is needed to prevent bumping. Stationary phase solubility vs. solvent composition is also briefly discussed. |
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| Keywords: | Static coating of capillary columns Solvent mixtures Coating speed Coating solution bumping Stationary phases solubility |
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