Identification of organic colourants in cosmetics by HPLC-diode array detection |
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| Authors: | S C Rastogi V J Barwick S V Carter |
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| Institution: | (1) Ministry of Environment and Energy, National Environmental Research Institute, Department of Environmental Chemistry, P.O. Box 358, 4000 Roskilde, Denmark;(2) Laboratory of the Government Chemist, Queens Road, TW11 OLY Teddington, Middlesex, UK |
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| Abstract: | Summary A method for the analysis of organic cosmetic colourants was developed. The colourants extracted from the cosmetic products
were analysed by HPLC followed by diode-array detection across the wavelength range 275 nm–760 nm. The chromatographic separation
was performed on a column with a polymeric packing using gradient elution with a mobile phase composed of citrate buffer,
the ion-pairing reagent tetrabutylammonium hydroxide, acetonitrile and tetrahydrofuran. The colourants were identified on
the basis of their retention times as well as their UV-visible spectra. A spectral library consisting of retention times and
UV-visible spectra of 130 organic cosmetic colourants has been built for the purpose of identifying of colouring matter in
cosmetic products. Solid-phase extraction methods using C-18 silica and aminobonded silica have been developed for the extraction
of colourants from various types of cosmetic products. The method for the identification of colourants in nail varnishes and
lipsticks has been optimized for routine analysis.
Presented at the 21st ISC held in Stuttgart, Germany, 15th–20th September, 1996 |
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| Keywords: | Column liquid chromatography Solid phase extraction Diode array detection UV-visible spectral library Cosmetics Colourants |
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