Determination of Methylene Blue and Its Metabolite Residues in Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry |
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| Authors: | Xuan Zhang Yunhua Hui Changling Fang Yuan Wang Feng Han Xiaoyi Lou Essy Kouadio Fodjo Youqiong Cai Cong Kong |
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| Institution: | 1.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; (X.Z.); (Y.H.); (C.F.); (Y.W.); (F.H.); (X.L.);2.Key Laboratory of East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;3.Physical Chemistry Laboratory, UFR SSMT, Université Felix Houphouet Boigny, Abidjan 22 BP 582, Côte d’Ivoire; |
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| Abstract: | A sensitive and reliable method was developed to determine methylene blue (MB) and its metabolite residues, including azure A (AZA), azure B (AZB), and azure C (AZC) in aquatic products by HPLC–MS/MS. The samples were extracted by acetonitrile and cleaned up by alumina-neutral (ALN) cartridges. The analytes were separated on a Sunfire C18 column (150 mm × 2.1 mm, 5 µm). The method was validated according to the European criteria of Commission Decision 2002/657/CE. Good linearity between 1–500 µg/L was obtained with correlation coefficients (R2) greater than 0.99. The limit of quantification (LOQ) was 1.0 µg/kg. The average recoveries at three levels of each compound (1, 5, and 10 µg/kg) were demonstrated to be in the range of 71.8–97.5%, with relative standard deviations (RSDs) from 1.05% to 8.63%. This method was suitable for the detection of methylene blue and its metabolite residues in aquatic products. |
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| Keywords: | high-performance liquid chromatography– tandem mass spectrometry methylene blue disinfectant aquatic products |
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