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| 钌(Ⅱ)多吡啶配合物与DNA相互作用的等温滴定量热研究(英) | |
| 引用本文： | 徐宏,张鹏,杜芬,周兵瑞,梁毅,刘剑洪,刘志刚,计亮年. 钌(Ⅱ)多吡啶配合物与DNA相互作用的等温滴定量热研究(英)[J]. 无机化学学报, 2005, 21(4): 468-471 |
| 作者姓名： | 徐宏 张鹏 杜芬 周兵瑞 梁毅 刘剑洪 刘志刚 计亮年 |
| 作者单位： | 1. 武汉大学生命科学学院,病毒学国家重点实验室,武汉,430072;深圳大学师范学院化生教育系,深圳,518060;南京大学配位化学国家重点实验室,南京,210093 2. 武汉大学生命科学学院,病毒学国家重点实验室,武汉,430072 3. 深圳大学师范学院化生教育系,深圳,518060 4. 中山大学化学与化工学院,广州,510275 |
| 基金项目： | 国家自然科学基金(No.30370309,90408012)，国家 863 计划(No.2004AA404260)，中国博士后科学基金(No.2003034503)，南京大学配位化学国家重点实验室开放课题资助项目。 |
| 摘 要： | The interaction of ruthenium(Ⅱ) polypyridyl complex with DNA has been studied by isothermal titration calorimetry (ITC). The results show that complex [Ru(phen)2PMIP]2+ {phen=1,10-phenanthroline, PMIP=2-(4-methylphenyl)imidazo[4,5-f]1,10-phenanthroline} interacts with calf thymus DNA (CT DNA) in terms of a model for a single set of identical sites through intercalation. The results are in agreement with our previous observations from spectroscopic methods and viscosity measurements. In addition, the results further show that the driving force for DNA binding with the complex is mainly driven by the enthalpy changes, and the contribution from the entropy changes to this driving force is negligible. |
| 关 键 词： | 钌(Ⅱ)多吡啶配合物； CT DNA； ITC； 相互作用 |
| 收稿时间： | 2004-11-11 |
| 修稿时间： | 2005-01-30 |
Studies on Interaction of Ruthenium(Ⅱ) Polypyridyl Complex with DNA Using Isothermal Titration Calorimetry |
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| XU Hong,ZHANG Peng,DU Fen,ZHOU Bing-Rui,LIANG Yi,LIU Jian-Hong,LIU Zhi-Gang and JI Liang-Nian. Studies on Interaction of Ruthenium(Ⅱ) Polypyridyl Complex with DNA Using Isothermal Titration Calorimetry[J]. Chinese Journal of Inorganic Chemistry, 2005, 21(4): 468-471 | |
| Authors: | XU Hong ZHANG Peng DU Fen ZHOU Bing-Rui LIANG Yi LIU Jian-Hong LIU Zhi-Gang JI Liang-Nian |
| Affiliation: | National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072;Department of Chemistry and Biology, Normal College, Shenzhen University, Shenzhen 518060;State Key Laboratory of Coordination Chemistry, Nanjing University, Nan,National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072,National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072,National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072,National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072,Department of Chemistry and Biology, Normal College, Shenzhen University, Shenzhen 518060,Department of Chemistry and Biology, Normal College, Shenzhen University, Shenzhen 518060 and School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510275 |
| Abstract: | The interaction of ruthenium polypyridyl complex with DNA has been studied by isothermal titration calorimetry (ITC). The results show that complex [Ru(phen)2PMIP]2 {phen=1,10-phenanthroline, PMIP=2-(4-methylphenyl)imidazo1,10-phenanthroline} interacts with calf thymus DNA (CT DNA) in terms of a model for a single set of identical sites through intercalation. The results are in agreement with our previous observations from spectroscopic methods and viscosity measurements. In addition, the results further show that the driving force for DNA binding with the complex is mainly driven by the enthalpy changes, and the contribution from the entropy changes to this driving force is negligible. |
| Keywords: | Ruthenium polypyridyl complexes CT DNA ITC Interaction |
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