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In vitro screening of the action of non-steroidal anti-inflammatory drugs on hypochlorous acid-induced hyaluronan degradation |
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| Authors: | Monika Stankovská ,Jozef Rychlý ,Peter Gemeiner |
| Affiliation: | a Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovak Republic b University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, D-04107 Leipzig, Germany c Polymer Institute, Department of Thermooxidation Degradation of Polymers, CEDEBIPO, Centre of Excellence for Degradation of Biopolymers, Slovak Academy of Sciences, Dubravska cesta 9, SK-84236 Bratislava, Slovak Republic d Institute of Chemistry, Slovak Academy of Sciences, SK-84538 Bratislava, Slovak Republic |
| Abstract: | The antioxidative effect of two non-steroidal anti-inflammatory drugs was studied in vitro by measuring the kinetics of degradation of high-molecular weight hyaluronan (HA) in a system comprising hypochlorous acid + CuCl2 + ascorbic acid using a Brookfield rotational viscometer equipped with a Teflon cup and spindle of coaxial cylindrical geometry. The changes in HA chemical structure were investigated by chemiluminometry. When sodium naproxen was added to the system during a running degradative process its inhibitory effect was clearly shown. The inhibition was dependent on the drug concentration. However, when this drug was added to the system before the initiation of HA degradation, no inhibition was seen even at the highest drug concentration tested. The inhibitory effect of acetylsalicylic acid was achieved with a relatively low concentration of the drug and was independent of the experimental model used. |
| Keywords: | Hyaluronan degradation Hypochlorous acid Non-steroidal anti-inflammatory drugs Rotational viscometry Chemiluminometry |
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