Separation of malignant human breast cancer epithelial cells from healthy epithelial cells using an advanced dielectrophoresis-activated cell sorter (DACS) |
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Authors: | Jaemin An Jangwon Lee Sang Ho Lee Jungyul Park Byungkyu Kim |
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Institution: | (1) School of Aerospace and Mechanical Engineering, Korea Aerospace University, Goyang, Gyeonggi-do, 412-791, Korea;(2) School of Life Sciences and Biotechnology, Korea University, 5ga-1, Anam-dong, Seongbuk-gu, Seoul, 136-701, Korea;(3) Mechanical Engineering, Sogang University, Sinsu-dong Mapo-gu, Seoul, 121-742, Korea |
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Abstract: | In this paper, we successfully separated malignant human breast cancer epithelial cells (MCF 7) from healthy breast cells
(MCF 10A) and analyzed the main parameters that influence the separation efficiency with an advanced dielectrophoresis (DEP)-activated
cell sorter (DACS). Using the efficient DACS, the malignant cancer cells (MCF 7) were isolated successfully by noninvasive
methods from normal cells with similar cell size distributions (MCF 10A), depending on differences between their material
properties such as conductivity and permittivity, because our system was able to discern the subtle differences in the properties
by generating continuously changed electrical field gradients. In order to evaluate the separation performance without considering
size variations, the cells collected from each outlet were divided into size-dependent groups and counted statistically. Following
that, the quantitative relative ratio of numbers between MCF 7 and MCF 10A cells in each size-dependent group separated by
the DEP were compared according to applied frequencies in the range 48, 51, and 53 MHz with an applied amplitude of 8 Vpp. Finally, under the applied voltage of 48 MHz–8 Vpp and a flow rate of 290 μm/s, MCF 7 and MCF 10A cells were separated with a maximum efficiency of 86.67% and 98.73% respectively.
Therefore, our suggested system shows it can be used for detection and separation of cancerous epithelial cells from noncancerous
cells in clinical applications. |
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Keywords: | Dielectrophoresis Cell separation Microfluidics Cancer Malignant cells |
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