Guanidinoamidized linear polyethyleneimine for gene delivery |
| |
Authors: | Bo Zhang Xin-peng Ma Mei-hua Sui Edward Van Kirk William J. Murdoch Maciej Radosz Neng-ming Lin You-qing Shen |
| |
Affiliation: | 1. Institute for Individualized Medicine, Hangzhou First People's Hospital Hangzhou 310006, China;Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital Hangzhou 310022, China;Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071, USA;2. Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071, USA;3. Center for Bionanoengineering and the State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;4. Department of Animal Science, University of Wyoming,Laramie, Wyoming 82071, USA;5. Institute for Individualized Medicine, Hangzhou First People's Hospital Hangzhou 310006, China |
| |
Abstract: | Guanidine was introduced to low molecular weight linear polyethyleneimine(LPEI) via amide groups, to explore the effect of both guanidine degree and pendant chain length on its transfection behavior. The resulting guanidinoamidized LPEIs(GLPEIs) could dramatically reduce LPEI's toxicity, enhance its DNA-packaging capability, cellular uptake and therefore transfection efficiency. These polyplexes were taken up very efficiently via caveolae-mediated endocytosis and their transfection efficiencies in ovarian cancer cells were significantly improved compared to native LPEI10 k polyplexes. Among these GLPEIs, LPEI-C3-G100 showed higher DNA affinity even than LPEI25 k and the highest transfection efficiency, probably due to the optimization of polymer chain flexibility. Of notice, LPEI-C3-G100 polyplexes could more effectively accumulate into cytoplasm than LPEI25 k, although the transfection efficiency of LPEI-C3-G100 polyplexes was not superior to that of LPEI25 k polyplexes, which would be probably attributed to the more efficient release of LPEI25 k polyplexes than LPEI-C3-G100 polyplexes in the cytoplasm. |
| |
Keywords: | Polyethyleneimine Guanidine Gene delivery Cellular uptake |
本文献已被 CNKI 万方数据 SpringerLink 等数据库收录! |
|