高效液相色谱法测定饲料中辛硫磷残留量

提出了高效液相色谱法测定饲料中辛硫磷残留量的方法。样品经乙酸乙酯提取,氨基固相萃取小柱净化,所得净化液以反相C18色谱柱为分离柱,以乙腈-水(65+35)混合溶液为流动相,在检测波长280nm处进行测定。辛硫磷的质量浓度与其峰面积在0.10～0.50mg·L-1范围内呈线性关系,方法的检出限(3S/N)为0.10mg·kg-1。加标回收率在80.0%～88.2%之间,测定值的相对标准偏差(n=5)在1.6%～6.5%之间。

HPLC Determination of Residual Amount of Phoxim in Feed

HPLC was applied to the determination of residual amount of phoxim in feed.The sample was extracted with ethyl acetate and purified by passing through NH2-solid phase extraction column.Reverse C18 chromatographic column was used as stationary phase,and a mixed solution of acetonitrile and water(65+35) was used as mobile phase.UV-detection at 280 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of phoxim was obtained in the range of 0.10-0.50 mg·L-1,with detection limit of 0.10 mg·kg-1.Values of recovery found by standard addition method were in the range of 80.0%-88.2%,and values of RSD′s(n=5) in the range of 1.6%-6.5%.