| 分离尿激酶的胍基型亲和色谱填料研究 |
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| 引用本文： | 常建华,高俊萍,梁峰,冯越,董聿生,苏天升,郭立安.分离尿激酶的胍基型亲和色谱填料研究[J].高等学校化学学报,2000,21(5):737-739. |
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| 作者姓名： | 常建华 高俊萍 梁峰 冯越 董聿生 苏天升 郭立安 |
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| 作者单位： | 1. 西北大学化学系, 西安 710069;
2. 中国科学院化学研究所, 北京 100080;
3. 第四军医大学中心实验室, 西安 710032 |
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| 基金项目： | 陕西省教育委员会重点科研项目!（批准号：96JZK15） |
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| 摘 要： | 尿激酶的精制一般使用亲和色谱法［’－‘ｊ．目前实际使用和文献报道最多的填料是在 ＳｅＰｈａｒｏｓｅ上键合对氨基苯眯（ｐ－ＡＢＺ）制得的［‘ｊ．这种填料分高效果较好，但机械强度不高，只能用于常压色谱，而且寿命较短．前文［‘１报道了用 ＳｅＰｈａｒｏｓｅ和聚甲基丙烯酸环氧丙酯微球为基质， Ｐ－ＡＢＺ为配基分离尿激酶的亲和色谱填料的对照研究．本文报道以含肥基的有机小分子为配基，ＳｅＰｈａｒｏｓｅ及两种聚甲基丙烯酸环氧丙酯微球为基质的分离尿激酶色谱填料的合成和性能试验．发现肥基己酸和精氨酸为配基的亲和色谱…
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| 关 键 词： | 填料 亲和色谱 分离 尿激酶 |
| 收稿时间： | 1999-07-13 |
Studies on the Packings with Guanido of Affinity Chromatography for the Separation of Urokinase |
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| CHANG Jian-Hua,GAO Jun-Ping,LIANG Feng,FENG Yue,DONG Yu-Sheng,SU Tian-Sheng,GUO Li-An.Studies on the Packings with Guanido of Affinity Chromatography for the Separation of Urokinase[J].Chemical Research In Chinese Universities,2000,21(5):737-739. |
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| Authors: | CHANG Jian-Hua GAO Jun-Ping LIANG Feng FENG Yue DONG Yu-Sheng SU Tian-Sheng GUO Li-An |
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| Institution: | 1. Department of Chemistry, Northwest University, Xian 710069, China;
2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
3. Central Laboratory, the Fourth Military University, Xi'an 710032, China |
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| Abstract: | Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine derivative is a fine ligand, the packings with ligand of arginine are much better than that with ligand of p-ABZ. The polyepoxypropyl methacrylate microsphere is a good matrix. The comprehensive function of packings with ligand of arginine and matrix of polyepoxypropyl methacrylate microsphere is more excellent than Sepharose-p-ABZ. |
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| Keywords: | Packings Affinity chromatography Separation Urokinase |
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