| Determination of Active Ingredients of Hawthorn by Capillary Electrophoresis with Electrochemical Detection |
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| 引用本文： | 唐祝兴 曾祎琨 周韵 臧树良 何品刚 方禹之. Determination of Active Ingredients of Hawthorn by Capillary Electrophoresis with Electrochemical Detection[J]. 中国化学, 2006, 24(10): 1378-1383. DOI: 10.1002/cjoc.200690257 |
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| 作者姓名： | 唐祝兴 曾祎琨 周韵 臧树良 何品刚 方禹之 |
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| 作者单位： | Department of Chemistry East China Normal University，Shanghai 200062，China Department of Chemistry，Shenyang Institute of Technology，Shenyang，Liaoning 110168，China，Department of Chemistry，East China Normal University，Shanghai 200062，China，Department of Chemistry，East China Normal University，Shanghai 200062，China，Department of Chemistry，Liaoning University，Shenyang，Liaoning 110036，China，Department of Chemistry，East China Normal University，Shanghai 200062，China，Department of Chemistry，East China Normal University，Shanghai 200062，China |
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| 基金项目： | Project supported by the Natural Science Research Foundation from Shanghai Science and Technology Committee(No.04ZR14041). |
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| 摘 要： | A method based on capillary electrophoresis with electrochemical detection has been developed for the separa-tion and determination of epicatechin,kaempferol,chlorogenic acid,4-hydroxybenzoic acid,quercetin and proto-catechuic acid in hawthorn for the first time.The effects of working electrode potential,pH and concentration ofrunning buffer,separation voltage and injection time on CE-ED were investigated.Under the optimum conditions,the analytes could be separated in a 60 mmol·L~(-1) borate buffer(pH 8.7)within 21 min.A 300 μm diameter carbondisk electrode has a good response at 0.95 V(vs.SCE)for all analytes.The response was linear over three ordersof magnitude with detection limits(S/N=3)ranging from 3×10~(-8) to 2×10~(-7) g·mL~(-1) for the analytes.The methodhas been successfully applied to the analysis of real sample,with satisfactory results.
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| 关 键 词： | 毛细管电泳分析 电气化学检测 山楂 活性成分 |
| 收稿时间： | 2006-03-27 |
| 修稿时间： | 2006-03-272006-06-02 |
Determination of Active Ingredients of Hawthorn by Capillary Electrophoresis with Electrochemical Detection |
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| TANG Zhu-Xing,ZENG Yi-Kun, ZHOU Yun, ZANG Shu-Liang, HE Pin-Gang, FANG Yu-Zhi. Determination of Active Ingredients of Hawthorn by Capillary Electrophoresis with Electrochemical Detection[J]. Chinese Journal of Chemistry, 2006, 24(10): 1378-1383. DOI: 10.1002/cjoc.200690257 |
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| Authors: | TANG Zhu-Xing ZENG Yi-Kun ZHOU Yun ZANG Shu-Liang HE Pin-Gang FANG Yu-Zhi |
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| Abstract: | A method based on capillary electrophoresis with electrochemical detection has been developed for the separation and determination of epicatechin, kaempferol, chlorogenic acid, 4‐hydroxybenzoic acid, quercetin and protocatechuic acid in hawthorn for the first time. The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CE‐ED were investigated. Under the optimum conditions, the analytes could be separated in a 60 mmol·L?1 borate buffer (pH 8.7) within 21 min. A 300 μm diameter carbon disk electrode has a good response at +0.95 V (vs. SCE) for all analytes. The response was linear over three orders of magnitude with detection limits (S/N=3) ranging from 3×10?8 to 2×10?7 g·mL?1 for the analytes. The method has been successfully applied to the analysis of real sample, with satisfactory results. |
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| Keywords: | capillary electrophoresis electrochemical detection hawthorn |
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