| 刺囊毛霉对甘草次酸的微生物转化研究 |
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| 引用本文： | 马晶,辛秀兰,张薇,兰蓉,刘俊英.刺囊毛霉对甘草次酸的微生物转化研究[J].北京化工大学学报(自然科学版),2008,35(1):79-82. |
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| 作者姓名： | 马晶 辛秀兰 张薇 兰蓉 刘俊英 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029;北京电子科技职业学院生物技术应用中心,北京,100029;北京电子科技职业学院生物技术应用中心,北京,100029 |
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| 基金项目： | 北京市属市管高校人才强教计划 |
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| 摘 要： | 对刺囊毛霉AS3.3450微生物转化甘草次酸的条件进行了研究。结果表明，生成的主产物，经分析鉴定是7β-羟基甘草次酸；最佳转化条件为培养温度27℃，底物加量为80mg/L，转化10d，主产物得率为875mg/g甘草次酸。刺囊毛霉AS3.3450能够专一性地对甘草次酸进行羟基化反应，这为研究甘草次酸药物开发及体内代谢研究提供一定的理论依据。
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| 关 键 词： | 甘草次酸 刺囊毛霉 微生物转化 |
| 收稿时间： | 2007-09-21 |
| 修稿时间： | 2007年9月21日 |
Microbial transformation of glycyrrhetinic acid by Mucor spinous |
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| MA Jing,XIN XiuLan,ZHANG Wei,LAN Rong,LIU JunYing.Microbial transformation of glycyrrhetinic acid by Mucor spinous[J].Journal of Beijing University of Chemical Technology,2008,35(1):79-82. |
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| Authors: | MA Jing XIN XiuLan ZHANG Wei LAN Rong LIU JunYing |
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| Institution: | 1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029;2. Biotechnology Application Center, Beijing Vocational College of Science and Technology, Beijing 100029, China |
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| Abstract: | The major product of microbial transformation of glycyrrhetinic acid by Mucor Spinosus AS3.3450 has been identified as 7β-hydroxyglycyrrhetinic acid on the basis of its spectral data. The optimum conditions for the biotransformation found to be: incubation temperature of 27℃, substrate concentration of 80 mg/L, and incubation time of 10 d. The maximum transformation rate observed was 875 mg/g. 7β-hydroxyglycyrrhetinic acid/g glycyrrhetinic acid on the 10th day. The knowledge that Mucor Spinosus AS3.3450 hydroxylates glycyrrhetinic acid in a site-specific position is important for future studies of the metabolism of glycyrrhetinic acid in vivo. |
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| Keywords: | glycyrrhetinic acid Mucor spinous microbial transformation |
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