Analytical Research Center, Production Technology Research Laboratories, Daiichi Pharmaceutical Co., Ltd, 1-16-13, Kitakasai Edogawa-ku, Tokyo 134, Japan
Abstract:
A method for the optical resolution of new quinolone drugs (NQs) by capillary electrophoresis has been investigated. The NQs were adequately resolved using a γ-cyclodextrin (γ-CD)-Zn(II)-
-phenylalanine (
-Phe) solution as the running solution. The resolution depended on the components of the running solution and their concentrations. When
-Phe was used instead of
-Phe, inversion of the migration order of the enantiomers was observed. The resolution mechanism is considered to be due to a ligand-exchange interaction of the NQs with Zn(II) and
-Phe, in combination with a host-guest interaction of those with γ-CD.