Linewidth-Resolved N HSQC, a Simple 3D Method to Measure N Relaxation Times from T1 and T2 Linewidths |
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| Authors: | Sami Heikkinen,Ilkka Kilpel inen |
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| Affiliation: | a Department of Radiology, Helsinki University Central Hospital, P.O. Box 340, HUCH, FIN-00029, Finland;b Institute of Biotechnology, NMR Laboratory, University of Helsinki, P.O. Box 56, FIN-00014, Finland |
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| Abstract: | A three-dimensional approach for measuring 15N relaxation times is described. Instead of selecting particular values for the relaxation period, in the proposed method the relaxation period is incremented periodically in order to create a 3D spectrum. This additional frequency domain of the transformed spectrum contains the relaxation time information in the T1 and T2 linewidths, and thus the longitudinal and transverse 15N relaxation times can be measured without determination of 2D cross peak volumes/intensities and subsequent curve fitting procedures. |
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| Keywords: | Abbreviations: relaxationAbbreviations: proteinAbbreviations: 3D experimentAbbreviations: linewidthAbbreviations: ubiquitinAbbreviations: 15N HSQC |
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