| Pseudomonas stutzeri 1317合成中长链聚羟基脂肪酸酯的组成分析模型 |
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| 引用本文： | 徐军,贺文楠,郭宝华,胡平,张增民,张广,林会兰,陈金春,陈国强.Pseudomonas stutzeri 1317合成中长链聚羟基脂肪酸酯的组成分析模型[J].高等学校化学学报,2002,23(1):71-74. |
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| 作者姓名： | 徐军 贺文楠 郭宝华 胡平 张增民 张广 林会兰 陈金春 陈国强 |
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| 作者单位： | 1. 清华大学材料科学与工程研究院, 化工系高分子研究所, 北京 100084;
2. 清华大学生物科学与技术系, 北京 100084 |
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| 基金项目： | 国家“九五”攻关基金 (批准号 :96 -C0 3-0 3-0 2 )，清华大学基金资助 |
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| 摘 要： | Pseudomonasstutzeri 1317可以在不同碳源上生长,合成不同共聚单体单元组成的中长链聚羟基脂肪酸酯(mclPHAs).本文在已知的代谢途径的基础上,提出了关于mclPHAs组成的数学模型,结果表明,mclPHAs的共聚单体单元组成主要取决于前体生成途径,以及该途径和聚合途径酶对不同碳原子数前体的选择性.实验结果与文献报道结果均验证了该模型.代谢控制分析的结果表明,碳数为8～10的前体的聚合效率最高.通过调制前体生成途径,可以有效调节PHA的单体单元组成.
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| 关 键 词： | 中长链聚羟基脂肪酸酯(mclPHAs) Pseudom onas stutzeri 数学模型 单体单元组成 代谢控制分析 |
| 文章编号： | 0251-0790(2002)01-0071-04 |
| 收稿时间： | 2000-06-27 |
A Mathematical Model for Biosynthesis of Medium-chain-length Polyhydroxyalkanoates by Pseudomonas stutzeri 1317 |
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| XU Jun,HE Wen-Nan,GUO Bao-Hua,HU Ping,ZHANG Zeng-Min,ZHANG Guang,LIN Hui-Lan,CHEN Jin-Chun,CHEN Guo-Qiang.A Mathematical Model for Biosynthesis of Medium-chain-length Polyhydroxyalkanoates by Pseudomonas stutzeri 1317[J].Chemical Research In Chinese Universities,2002,23(1):71-74. |
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| Authors: | XU Jun HE Wen-Nan GUO Bao-Hua HU Ping ZHANG Zeng-Min ZHANG Guang LIN Hui-Lan CHEN Jin-Chun CHEN Guo-Qiang |
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| Institution: | 1. Institute of Polymer Science & Engineering, Department of Chemical Engineering, School of Materials Science and Engineering, Tsinghua Unviversity, Beijing 100084, China;
2. Department of Biological Science & Technology, Tsinghua University, Beijing 100084, China |
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| Abstract: | When grown on various carbon sources, P. stutzeri 1317 strain accumulates medium chain length polyhydroxyalkanoates(mcl PHAs) of various monomer composition. A mathematical model was developed to investigate what affects mcl PHA composition and how to tailor design the copolymer, and some interesting conclusions were deduced: the monomer composition was independent of cultivation time and the enzyme level; the content variance of different monomers resulted only from the selectivity ratio of the polymerase to the enzymes involved in theprecursor formation pathway; modulation of precursor formation pathway led to convenient manipulation of PHA monomer composition. The experimental results from several Pseudomonas strains proved the model an effective tool for tailor design of mcl PHA copolymers. |
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| Keywords: | Medium chain length polyhydroxyalkanoates(mcl PHAs) Pseudomonas stutzeri Mathematical model Monomer composition Metabolic control analysis |
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