Evaluation of inoculum of Candida guilliermondii grown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate |
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Authors: | Débora Danielle Virgínio da Silva Maria das Graças de Almeida Felipe Ismael Maciel de Mancilha Sílvio Silvério da Silva |
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Affiliation: | 1. Department of Biotechnology, Faculty of Chemical Engineering of Lorena, FAENQUIL, Rodovia Itajuba-Lorena, Km 74.5, Caixa Postal 116, 12600-970, Lorena, SP, Brazil 2. Department of Food Technology, UFV, 06691-ax, Vi?osa, MG, Brazil
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Abstract: | The effect of glucose on xylose-xylitol metabolism in fermentation medium consisting of sugarcane bagasse hydrolysate was evaluated by employing an inoculum of Candida guilliermondii grown in synthetic media containing, as carbon sources, glucose (30 g/L), xylose (30 g/L), or a mixture of glucose (2 g/L) and xylose (30 g/L). The inoculum medium containing glucose promoted a 2.5-fold increase in xylose reductase activity (0.582 IU/mgprot) and a 2-fold increase in xylitol dehydrogenase activity (0.203 IU/mgprot) when compared with an inoculum-grown medium containing only xylose. The improvement in enzyme activities resulted in higher values of xylitol yield (0.56 g/g) and productivity (0.46 g/[L·h]) after 48 h of fermentation. |
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Keywords: | Xylose reductase xylitol dehydrogenase glucose Candida guilliermondii sugarcane bagasse hydrolysate |
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