自组装短肽作为“实时监控”纳米材料的初步研究

本文主要采用圆二色谱仪、原子力显微镜、刚果红染色等来探究手性自组装短肽GFS-15的二级结构、纳米形态和宏观形貌特征;通过循环伏安曲线、圆二色谱来检测浓度、时间、盐离子对它自组装过程的影响。结果表明,GFS-15能形成纳米纤维,能和金电极结合且影响其电化学行为,还可以作为细胞的三维培养基质材料;细胞三维培养常规实验以及细胞三维培养实时监控实验表明,细胞可在该肽形成的纳米三维微环境中生长,也能组装在金电极传感器的(纳米金)表面。本研究将为生物实时监控以及纳米生物医学的前沿研究打下基础。

Preliminary study to monitor real-time by using self-assembling peptide nanomaterials

The secondary structure, nanostructure and macro-feature to peptide GFS-15 were characterized by circular dichroism spectroscopy (CD), atomic force microscopy (AFM) and Congo red dye. We adopted cyclic voltammetry and CD to study the influence of concentration, self-assembly time and ions to the self-assembly process of the peptide. The result shows that this peptide can form nano-fiber, and the nano-fiber can bind to gold electrode, form to the hydrogel support 3D cell culture matirx. 3D cell culture test, and 3D cell culture real-time tests, confirmed the cell strain not only growth in the nano 3D microenvironment, but also self-assembling to gold electrode sensor (nano-gold particles) surface. Our study could inspire cell real-time monitor and nano-biomedical developing.