| PLS分光光度法同时测定混合氨基酸 |
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| 引用本文： | 王雁鹏,董旭辉,陈岩,董树屏.PLS分光光度法同时测定混合氨基酸[J].光谱实验室,2006,23(5):1123-1126. |
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| 作者姓名： | 王雁鹏 董旭辉 陈岩 董树屏 |
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| 作者单位： | 国家环境分析测试中心,北京市,100029;国家环境分析测试中心,北京市,100029;国家环境分析测试中心,北京市,100029;国家环境分析测试中心,北京市,100029 |
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| 摘 要： | 本文将偏最小二乘法(PLS)用于分光光度法同时测定混合氨基酸体系中的苯丙氨酸、色氨酸和酪氨酸.对校准样品集的实验设计及测量波长点的选择等进行了实验和讨论,并与最小二乘法、卡尔曼滤波法和因子分析法的计算结果进行了对比,PLS法的计算结果最精确,偏差最小.由此可见,PLS法对于这类组分间存在相互作用的样品的测定是一种简捷、准确的多组分同时测定方法,通过校准集来校正相互作用带来的影响,是PLS法偏差最小的原因.
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| 关 键 词： | 偏最小二乘 氨基酸 分光光度法 |
| 文章编号： | 1004-8138(2006)05-1123-04 |
| 收稿时间： | 2006-09-06 |
| 修稿时间： | 2006-09-11 |
Simultaneous Determination of Mixed Amino Acids by PLS Spectrophotometry |
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| WANG Yan-Peng,DONG Xu-Hui,CHEN Yan,DONG Shu-Ping.Simultaneous Determination of Mixed Amino Acids by PLS Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2006,23(5):1123-1126. |
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| Authors: | WANG Yan-Peng DONG Xu-Hui CHEN Yan DONG Shu-Ping |
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| Institution: | National Research Center for Environmental Analysis and Measurernents,Beijing 100029,P. R. China |
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| Abstract: | The partial least square (PLS) method for spectrophotometric simultaneous determination of phenylalanine,tryptophan and tyrosine in the mixed amino acids was studied.The orthogonal design in the calibration sample sets and the election of wavelength were discussed.The results of PLS are more accurate and reliable compared with those of least square (LS),Kalman filtering (KF) and factor Analysis (FA).All in all,the PLS analysis method can be used to determine accurately mixed amino acids which exhibits intrinsic interaction among components by appropriate calibration sample sets. |
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| Keywords: | PLS Spectrophotometry Amino Acid |
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