THE COMPLEXES FORMED BY BILADIENE a, b COMPOUNDS WITH ZINC IONS and THEIR APPLICATION IN DETERMINATION OF THE CHROMOPHORE COMPOSITION OF α- and β- SUBUNITS OF R-PHYCOERYTHRIN |
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| Authors: | Ling-Jiang Cheng Jin-Shi Ma Li-Chin Chiang |
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| Institution: | Institute of Photographic Chemistry, Academia Sinica, Beishatan, Dewai, Beijing 100101, China |
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| Abstract: | Abstract— –Phycoerythrobilin dimethyl ester (PEBDM) or isomesobiliviolin dimethyl ester (IBVDM) formed complexes with zinc ions in chloroform. The complex ratios were determined to be 1:1 according to the spectrophotometric titration of PEBDM and IBVDM with zinc ions in methanol and the mass spectra of the complexes. The chromophore compositions of R-phycoerythrin from Porphyra yezoensis were determined by spectrophotometric titration of α- and β-subunits at pH 13.0 with zinc ions. There were 2 phycoerythrobilins in the a-subunit, and 1 phycourobilin and 3 phycoerythrobilins in the β-subunit. |
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