石墨炉原子吸收法检测新生儿胎粪铅水平

用硝酸消化新生儿胎粪铅含量的石墨炉原子吸收法(GFAAS)测定了144例新生儿胎粪铅的含量。0.3g胎粪于60℃烤箱烘烤过夜,80℃水浴下以HNO3和H2O2顺序消化1h后定容至2mL。用D2灯校正背景,样本基体匹配标准曲线法测定。实验组93例胎粪样本,铅平均值为1.934μg.g-1,标准差(SD)为1.551;对照组51例样本,平均值为1.012μg.g-1,标准差(SD)为1.084。实验组平均值明显高于对照组,两组均值比较做t检验p=0.000,p<0.05,两组均值在统计学上有显著差异。所建立的胎粪铅检测方法稳定,简便可行。

Determination of Meconium Lead Level of Newborn by Graphite Furnace Atomic Absorption Spectrometry

To establish a method for the determination of lead in meconium from 144 samples of newborn through nitric acid digestion by means of graphite furnace atomic absorption spectrometry. METHODS: after being baked for at least 12 h at 60 degrees C, 0.3 g of meconium was digested by nitric acid and hydrogen peroxide in turn at 80 degrees C under water-bath condition for 1h and then metered to the whole volume of to 2 mL. After correcting the background with D2 lamp, specimen basal corpuscle matched standard curve was used to detect the lead content. RESULTS: The mean lead content of 93 experimental samples was 1.934 microg x g(-1) with the standard deviation (SD) of 1.551, and that of the 51 control samples was 1.012 microg x g(-1), with the SD of 1.084. There was a significant difference in lead levels of in meconnt between the experimental group and control group (p = 0.000). CONCLUSION: The lead content of the experimental was significantly higher than that of the control group detected by this method. This method was stable and efficient.