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| 小麦雌性不育基因的微卫星标记定位 | |
| 引用本文： | 李生强,窦秉德,杜金昆,许盛宝,曹俊梅,陈莉,孙其信.小麦雌性不育基因的微卫星标记定位[J].淮阴师范学院学报(自然科学版),2005,4(3):231-235. |
| 作者姓名： | 李生强 窦秉德 杜金昆 许盛宝 曹俊梅 陈莉 孙其信 |
| 作者单位： | 1. 淮阴师范学院,学物系,江苏,淮安,223300;新疆农业大学,农学院,新疆,乌鲁木齐,830052 2. 中国农业大学,农业与生物技术学院,北京,100094 3. 新疆农业大学,农学院,新疆,乌鲁木齐,830052 |
| 基金项目： | 国家自然科学基金资助项目（30060044）; 新疆自然科学基金资助项目（20007）. |
| 摘 要： | 以普通小麦新601×雌性不育小麦XND126的F2群体作为育性调查以及基因标记群体.通过对育性基因的分析,确定在此组合中雌性不育基因由1对主效基因控制;结合混合分组分析法(Bulk Segregant Analysis,BSA),首次对小麦雌性不育基因进行了SSR分子标记,通过对一千对微卫星引物的筛选,确定微卫星引物cfd36标记与主效基因连锁,遗传距离为20.2cM. |
| 关 键 词： | 小麦 雌性不育基因 微卫星 |
| 文章编号： | 1671-6876（2005）03-0231-05 |
| 收稿时间： | 2005-05-20 |
| 修稿时间： | 2005年5月20日 |
Location of the Sterile Gene for Female Sterile Wheat by Microsatellite Marker |
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| LI Sheng-qiang,DOU Bing-de,DU Jin-kun,XU Sheng-bao,CAO Jun-mei,CHEN Li,SUN Qi-xin.Location of the Sterile Gene for Female Sterile Wheat by Microsatellite Marker[J].Journal of Huaiyin Teachers College(Natrual Science Edition),2005,4(3):231-235. | |
| Authors: | LI Sheng-qiang DOU Bing-de DU Jin-kun XU Sheng-bao CAO Jun-mei CHEN Li SUN Qi-xin |
| Institution: | LI Sheng-qiang~ |
| Abstract: | A study was carried out through the genetic analysis of F_2 population which is derived from a common wheat (XIN601)and the female sterile line (XND126), the result indicated that the sterile was condition by a pair recessive gene in the cross. The F_2 population was used to mark the female sterile gene by microsatellite and BSA (bulked segregant analysis) at the first time. Among the 1,000 pairs of microsatellite primers, a marker was found polymorphic between the two pools and the two parents. Linkage analysis showed that microsatellite marker cfd36 was linked with the sterile gene, and the genetic distance between the SSR marker cfd36 and the sterile gene was 22.0?cM. |
| Keywords: | wheat female sterile gene microsatellite marker |
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