Sample stacking and sweeping in microemulsion electrokinetic chromatography under pH-suppressed electroosmotic flow |
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Authors: | Zhu Jinhua Qi Shengda Zhang Huige Chen Xingguo Hu Zhide |
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Affiliation: | State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China. |
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Abstract: | Two on-line sample concentration techniques, sample stacking and sweeping under pH-suppressed electroosmotic flow, were evaluated in microemulsion electrokinetic chromatography. The concept of stacking with anion selective electrokinetic injection and a water plug in a reverse-migrating microemulsion (SASIW-RMME) was brought forward in this article. Six flavonoids were concentrated using a microemulsion consisting of 80 mM sodium dodecyl sulfate, 1.2% (v/v) ethyl acetate, 0.6% (v/v) 1-butanol, 10% acetonitrile (v/v) and 50 mM phosphoric acid (pH* 1.8). Significant detector response improvements were achieved. The limits of detection were in the low ng/ml level. Finally, the sample of Fructus aurantii Immaturus was analyzed using sweeping technique. |
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