Dynamic properties of soy globulin adsorbed films at the air-water interface |
| |
Authors: | Rodríguez Patino Juan M Molina Ortiz Sara E Carrera Sánchez Cecilio Rodríguez Niño Maria Rosario Añón Maria Cristina |
| |
Affiliation: | Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, c. Prof. García González, s/núm. 41012, Sevilla, Spain. jmrodri@us.es |
| |
Abstract: | In this paper we present surface dilatational properties of soy globulins (beta-conglycinin, glycinin, and reduced glycinin with 10 mM of dithiothreitol (DTT)) adsorbed onto the air-water interface, as a function of adsorption time. The experiments were performed at constant temperature (20 degrees C), pH (8.0), and ionic strength (0.05 M). The surface rheological parameters were measured as a function of protein concentration (ranging from 1 to 1x10(-3)% wt/wt). We found that the surface dilatational modulus, E, increases, and the phase angle, phi, decreases with time, theta, which may be associated with protein adsorption. These phenomena have been related to protein adsorption, unfolding, and/or protein-protein interactions (at long-term adsorption) as a function of protein concentration in solution. From a rheological point of view, the surface viscoelastic characteristics of soy globulin films adsorbed at the air-water interface are practically elastic. The main conclusion is that the dilatational properties of the adsorbed films depend on the molecular structure of the protein. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|