Zur Totalsynthese von Human-Sekretin |
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Authors: | E Wünsch G Wendlberger W Göhring G Hübener B Traving |
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Institution: | (1) Max-Planck-Institut für Boichemie, Abteilung für Peptidchemie, D-8033 Martinsried, Bundesrepublik Deutschland |
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Abstract: | Summary The synthesis of the heptacosapeptide amide with the primary structure of Human-secretin is described. For this purpose 7 fragments were designed, i.e. H-Gly-Leu-Val-NH2 25–27b ,Z-Arg(Z
2)-Leu-Leu-Gln-OH 21–24 ,Z-Arg(Z
2)-Leu-Gln-OH 18–20 ,Z-Arg(Z
2)-Glu(OtBu)-Gly-Ala-OH 14–17 ,Z-Arg(Z
2)-Leu-OH 12–13 ,Z-Thr(tBu)-Ser(tBu)-Glu(OtBu)-Leu-Ser(tBu)-OH 7–11 ,Adoc-His(Adoc)-Ser(tBu)-Asp(OtBu)-Gly-Thr(tBu)-Phe-OH 1–6 these fragments were consequently assembled to the overall protected total sequence using the Wünsch/Weygand-method with dicyclohexylcarbodiimide. After deprotection by exposure to trifluoroacetic acid in presence of 1,2-ethanedithiol and water as scavenger, the isolated crude product was purified by column chromatography on CM-Sepharose, fast flow. This synthetized Human-secretin showed the full biological activity in comparison to Porcine-secretin.Herrn Prof. Dr. E. Bayer zum 65. Geburtstag gewidmet. |
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Keywords: | Gastrointestinal hormone Human-secretin Synthetic peptide factors |
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