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Spectrofluorimetric quantification of bilirubin using yttrium–norfloxacin complex as a fluorescence probe in serum samples |
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| Authors: | Mohammad Kamruzzaman Al-Mahmnur Alam Sang Hak Lee Young Ho Kim Gyu-Man Kim Sang Hyub Oh |
| Institution: | 1. Department of Chemistry, Kyungpook National University, Daegu 702-701, South Korea;2. Research Institute of Advanced Energy Technology, Kyungpook National University, Daegu 702-701, South Korea;3. School of Mechanical Engineering, Kyungpook National University, Daegu 702-701, South Korea;4. Center for Gas Analysis, Korea Research Institute of Standards and Science, Daejeon 305-600, South Korea |
| Abstract: | A simple and sensitive spectrofluorimetric method was developed to determine trace amounts of bilirubin (BR) using yttrium (Y3+)–norfloxacin (NFLX) complex as a fluorescence (FL) probe. NFLX can form a stable binary complex with Y3+ and markedly enhances the weak FL signal of the NFLX. The FL intensity of the Y3+–NFLX complex decreased significantly in the presence of BR in a buffer solution at pH=7.2. Under optimal conditions, the FL intensity decreased according to the BR concentration and showed a good linear relationship in the range of 0.03–2.3 μg mL?1 of BR with a correlation coefficient of 0.9988. The limit of detection for the determination of BR was 2.8 ng mL?1 with a relative standard deviation (RSD) of 1.55% for five replicate determination of 0.05 μg mL-1 BR. The presented method offers higher sensitivity with simple instrumentation and was applied successfully in detecting BR at low concentrations. |
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