Disposable Electrochemical Aptasensor for Ultrasensitive Determination of Aflatoxin B1 Using Copper Nanoparticles as Probes |
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| Authors: | Jiaxiang Liu Yanli Zhou Hui Dong Qiaoxia Li Yintang Zhang Maotian Xu |
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| Institution: | 1. College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai, 20090 P. R. China;2. Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000 P. R. China |
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| Abstract: | An electrochemical aptasensor for aflatoxin B1 (AFB1) detection was constructed based on the copper nanoparticles (CuNPs) and gold nanoflowers modified screen-printed carbon electrodes as electrochemical probes and substrates, respectively. In the range of 100 aM to 100 pM, a good linear relationship between oxidation peak current of CuNPs and concentration of AFB1 was obtained. The high sensitivity could be ascribed to the amplified electrochemical signals by CuNPs. The high affinity of AFB1 with aptamer endowed its high selectivity. The above advantages and disposable traits made this aptasensor as an ideal platform for evaluation of AFB1 level in food samples. |
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| Keywords: | Electrochemical aptasensor Aflatoxin B1 Copper nanoparticles Screen-printed carbon electrode Food analysis |
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