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Pyruvate Oxidase Biosensors Based on Glassy Carbon Electrodes Modified with Carbon Nanotubes and Poly(Neutral Red) Synthesized in Ethaline Deep Eutectic Solvent |
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| Authors: | Ricardo J. B. Leote Mariana E. Ghica Christopher M. A. Brett |
| Affiliation: | 1. University of Coimbra, CEMMPRE, Department of Chemistry, 3004-535 Coimbra, Portugal National Institute of Materials Physics, Atomistilor Str. 405 A, 077125 Magurele, Romania;2. University of Coimbra, CEMMPRE, Department of Chemistry, 3004-535 Coimbra, Portugal University of Coimbra, CIEPQPF, Department of Chemical Engineering, 3030-790 Coimbra, Portugal;3. University of Coimbra, CEMMPRE, Department of Chemistry, 3004-535 Coimbra, Portugal |
| Abstract: | A novel nanostructured platform for pyruvate oxidase biosensors comprises poly(neutral red) (PNR) prepared by electropolymerization of NR in ethaline deep eutectic solvent (DES) with acid dopant, on a multiwalled carbon nanotubes (MWCNT) modified glassy carbon electrode (GCE). Characterization was by cyclic voltammetry and electrochemical impedance spectroscopy and morphology was examined by scanning electron microscopy. Ascorbate and H2O2 gave a better response at PNRDES/GCE than at PNRaq/GCE. Biosensors for pyruvate and phosphate, immobilizing pyruvate oxidase onto PNRDES/MWCNT/GCE enabled selective determination of pyruvate and phosphate, with micromolar limits of detection. Pyruvate was determined in onion samples and phosphate in water samples. |
| Keywords: | Ethaline deep eutectic solvent poly(neutral red) MWCNT pyruvate oxidase phosphate |