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An Aptasensing Strategy Using the Phosphatase-mimic Nanozyme and pH Meter as Signal Readout |
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| Authors: | Xudong Shang Jingyuan Yu Chang Wang Yan Du |
| Affiliation: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 P. R. China University of Chinese Academy of Sciences, Beijing, 100039 P. R. China;2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 P. R. China University of Science and Technology of China, Hefei, 230026 P. R. China;3. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 P. R. China |
| Abstract: | The demand for point-of-care testing (POCT) is growing dramatically, especially for district where health facilities are poorly staffed, poorly skilled and ill-equipped. As a commercialized portable device, pH meters can be used for detection of various targets, relying on bioactive enzymes. The nanozymes, as the alternative of the natural enzymes, have rarely been used for pH-metric POCT strategies. Herein, we developed an ultrasensitive pH-metric sandwich-type aptasensor based on the CeO2 nanorods (CeO2 NRs) as phosphatase-mimic and sodium monofluorophosphate (MFP) as catalytic substrate. Under optimal conditions, such strategy yields a detection limit of 1.17 nM with eligible selectivity for detecting thrombin. |
| Keywords: | point-of-care testing (POCT) aptasensor nanozyme pH-metric biosensor nanoceria |