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Glucometer-based Ultra-sensitive BRAF V600E Mutation Detection Facilitated by Magnetic Nanochains and a Self-made Point-of-Care (POC) Device |
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| Authors: | Chang Wang Jingyuan Yu Lijuan Qi Jiaxue Yu Meiting Yang Yan Du |
| Institution: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 P. R. China
Department of Chemistry, University of Science & Technology of China, Hefei, Anhui, 230026 China;2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 P. R. China |
| Abstract: | We realized the user-friendly and field-based point-of-care detection on a self-made portable device with the aid of personal glucose meter (PGM) for single nucleotide polymorphisms (SNPs) of melanoma and colon cancer marker BRAF V600E with good sensitivity and selectivity by integrating the loop-mediated isothermal amplification (LAMP) reaction and the strand exchange signal transduction. The device is equipped with a rotating magnetic field as well as a heating element, under which the pre-added magnetic nanochains (MNCs) help to homogenize the reaction mixture and overcome the low diffusion efficacy of the reactant and the required temperature for different reactions can be met. |
| Keywords: | molecular recognition biosensors point-of-care testing (POCT) single nucleotide polymorphisms (SNPs) magnetic nanochains (MNCs) |