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Electrochemical Immunosensor for Lactate Dehydrogenase Detection Through Analyte-driven Catalytic Reaction on Multi-walled Carbon Nanotubes and Gold Nanoparticle Modified Carbon Electrode |
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| Authors: | Yuhan Yi Yi Li Weizhong Li Mingjie Cheng Meisheng Wu Jinfeng Miao Wei Kang Yuanyuan Xu |
| Affiliation: | 1. MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095 China This authors have contributed to this work equally.;2. MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095 China;3. Department of Chemistry, College of Science, Nanjing Agricultural University, Nanjing, 210095 China;4. Customs Technology Centre of Huangpu, No.66, Sanyuan Road, Nancheng District, Dongguan, 523073 China |
| Abstract: | A novel electrochemical immunosensor for lactate dehydrogenase (LDH) detection was proposed based on analyte-driven catalytic reaction by attaching LDH antibodies on multi-walled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) modified glassy carbon electrodes (GCE). As LDH was captured by the antibodies on electrode surface, it catalyzed the formation of pyruvate and the reduced form of nicotinamide adenine dinucleotide (NADH), thus a sensitive electrochemical signal obtained from the above redox reaction was recorded by differential pulse voltammetry (DPV). Under optimum conditions, the developed immunosensor exhibits high sensitivity for LDH quantification ranging from 0.001 μg/mL to 0.5 μg/mL with a low detection limit at 0.39 ng/mL. This developed immunosensor reveals ideal accuracy and feasibility for LDH detection in Streptococcus uberis (S. uberis) induced bovine mammary epithelial cells (MECs) samples by comparison with conventional commercial kit, which shows remarkably application potential in diseases diagnosis. |
| Keywords: | Streptococcus uberis mastitis mammary epithelial cells pyruvate nicotinamide adenine dinucleotide differential pulse voltammetry |