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A Sandwich-type Electrochemical Immunosensor for the Sensitive Determination of Salmonella Typhimurium in Food |
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| Authors: | Qiaoling Sun Xiaojun Liu Haowen Tang Yuying Qian Prof Haiying Gu Hong He |
| Institution: | 1. School of Public Health, Nantong University, 226019 Nantong, P.?R. China
These authors have contributed equally to this work and share first authorship.;2. Jiangyin Center for Disease Control and Prevention, 214431 Wuxi, P.?R. China These authors have contributed equally to this work and share first authorship.;3. School of Public Health, Nantong University, 226019 Nantong, P.?R. China;4. Affiliated Hospital, Nantong University, 226001 Nantong, P.?R. China |
| Abstract: | A sandwich-type electrochemical immunosensor was developed for sensitive detection of Salmonella Typhimurium (S. Typhimurium) in milk and eggs. According to the antigen-antibody specific binding, Anti-S. Typhimurium antibody was used to recognize the S. Typhimurium, while the HRP-Anti-S. Typhimurium antibody as signal probe was used to catalyze H2O2 with the assistance of thionine. The immunosensor showed wide linear range from 5.6×101 to 5.6×108 CFU/mL and low detection limit of 35 CFU/mL. The immunosensor also exhibited excellent specificity, reproducibility and stability, indicating that it had practicability in quality control of complex food samples and potential in clinical applications. |
| Keywords: | Electrochemical Immunosensor Food Control Sandwich-type Salmonella Typhimurium |