| Institution: | 1. Key Laboratory of Mass Spectrometry Imaging and Metabolomics, Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081 China
Center for Imaging and Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081 China;2. Key Laboratory of Mass Spectrometry Imaging and Metabolomics, Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081 China |
| Abstract: | Chemotherapy with cisplatin induces side effects such as memory loss, confusion of thinking, and difficulties with multi-tasking. However, the mechanism of cisplatin inducing nervous dysfunction is still unknown. Herein, we examine whether and how cisplatin regulates the release of neurotransmitter during exocytosis in single chromaffin cells using single cell amperometry. The results show that cisplatin reduces the amount of transmitter released during exocytosis by reducing the duration of the exocytotic events, including the opening and closing time of the fusion pore. Furthermore, the stability of the initial fusion pore formed during exocytosis is also reduced by cisplatin. Our study holds the promise for understanding the side effects of cisplatin on the nervous system at single cell level. |
