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Trapping and releasing of single microparticles and cells in a microfluidic chip |
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| Authors: | Dan Lv Xiaoling Zhang Mengli Xu Wenyue Cao Xing Liu Jinan Deng Jun Yang Ning Hu |
| Institution: | 1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing, P. R. China
These authors contributed equally to this work.;2. Chongqing School, University of Chinese Academy of Sciences, Chongqing, P. R. China;3. Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing, P. R. China |
| Abstract: | A microfluidic device was designed and fabricated to capture single microparticles and cells by using hydrodynamic force and selectively release the microparticles and cells of interest via negative dielectrophoresis by activating selected individual microelectrodes. The trap microstructure was optimized based on numerical simulation of the electric field as well as the flow field. The capture and selective release functions of the device were verified by multi-types microparticles with different diameters and K562 cells. The capture efficiencies/release efficiencies were 95.55% ± 0.43%/96.41% ± 1.08% and 91.34% ± 0.01%/93.67% ± 0.36% for microparticles and cells, respectively. By including more traps and microelectrodes, the device can achieve high throughput and realize the visual separation of microparticles/cells of interest in a large number of particle/cell groups. |
| Keywords: | hydrodynamic trapping negative dielectrophoresis particle manipulation single-cell analysis single-cell releasing |