| Institution: | 1. School of Pharmacy, Lanzhou University, Lanzhou, 730000 China
These authors contributed equally to this article;2. School of Pharmacy, Lanzhou University, Lanzhou, 730000 China;3. National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Liaocheng, China;4. Gansu Academy of Medical Science, Xiaoxihu East Street, Lanzhou, Gansu Province, 730050 China |
| Abstract: | The accurate and sensitive analysis of rheumatoid arthritis(RA) trace biomarkers is essential for early warning and diagnosis of RA, while anti-cyclic citrullinated peptide antibody (anti-CCP-ab) is a specific one. In this study, a simple label-free electrochemical immunosensor for the detection of anti-CCP-ab was constructed with nitrogen-doped graphene (N−G) and gold nanoparticles (AuNPs). The proposed non-enzyme immunosensor had exhibited high specificity, selectivity, and stability in the linear calibration curve range from 0.125∼2000 pg mL-1 and has been successfully used in the detection of anti-CCP-ab in human serum, providing a new idea for the early diagnosis of RA. |
