Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs |
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Authors: | Hana Mlčochová Ratih Ratih Lenka Michalcová Hermann Wätzig Zdeněk Glatz Matthias Stein |
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Affiliation: | 1. Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Lower Saxony, Germany Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic;2. Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Lower Saxony, Germany;3. Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic |
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Abstract: | In this study, two capillary electrophoresis–based ligand binding assays, namely, mobility shift affinity capillary electrophoresis (ms-ACE) and capillary electrophoresis-frontal analysis (CE-FA), were applied to determine binding parameters of human serum albumin toward small drugs under similar experimental conditions. The substances S-amlodipine (S-AML), lidocaine (LDC), l -tryptophan (l -TRP), carbamazepine (CBZ), ibuprofen (IBU), and R-verapamil (R-VPM) were used as the main binding partners. The scope of this comparative study was to estimate and compare both the assays in terms of their primary measure's precision and the reproducibility of the derived binding parameters. The effective mobility could be measured with pooled CV values between 0.55% and 7.6%. The precision of the r values was found in the range between 1.5% and 10%. Both assays were not universally applicable. The CE-FA assay could successfully be applied to measure the drugs IBU, CBZ, and LDC, and the interaction toward CBZ, S-AML, l -TRP, and R-VPM could be determined using ms-ACE. The average variabilities of the estimated binding constants were 64% and 67% for CE-FA and ms-ACE, respectively. |
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Keywords: | binding parameters capillary electrophoresis-frontal analysis drugs human serum albumin mobility shift affinity capillary electrophoresis |
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