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A passive pump-assisted microfluidic assay for quantifying endothelial wound healing in response to fluid shear stress
Authors:Yunong Yang  Yongjiang Li  Miao Yu  Chundong Xue  Bo Liu  Yanxia Wang  Kairong Qin
Institution:1. School of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian, Liaoning Province, P. R. China;2. School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian, Liaoning Province, P. R. China;3. School of Rehabilitation Medicine, Weifang Medical University, Weifang, Shandong Province, P. R. China
Abstract:There as an urgent need to quantify the endothelial wound-healing process in response to fluid shear stress to improve the biological and clinical understanding of healing mechanisms, which is of great importance for preventing healing impairment, chronic wounds, and postoperative in-stent restenosis. However, current experimental platforms not only require expensive, cumbersome, and powered pumping devices (to, e.g., generate cell scratches and load shear stress stimulation) but also lack quantitative controls for quantitative analysis. In this paper, a passive pump-assisted microfluidic assay is developed to quantify endothelial wound healing in response to fluid shear stress. Our assay consists of passive constant-flow pumps based on the siphon principle and a three-inlet microfluidic chip for cell wound-healing experiments. We also propose a method for quantitatively adjusting cell scratch size by controlling trypsin flow. Both numerical simulations and fluorescein experiments validate the effectiveness of this method. Moreover, we use the designed microfluidic assay to successfully generate cell scratches, load a 12-h shear stress of 5 dyn/cm2 to the cells, and observe wound healing. The results indicate that the healing of a cell scratch is significantly accelerated under the stimulation of shear stress. In conclusion, our passive pump-assisted microfluidic assay shows versatility, applicability, and the potential for quantifying endothelial wound healing in response to fluid shear stress.
Keywords:endothelial wound healing  microfluidics  passive pump  quantitative control of cell scratch size  shear stress
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