首页 | 本学科首页   官方微博 | 高级检索  
     


Alkaline Phosphatase-based Electrochemical Analysis for Point-of-Care Testing
Authors:Yusuke Kanno  Yuanshu Zhou  Takeshi Fukuma  Yasufumi Takahashi
Affiliation:1. Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8503 Japan.;2. Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192 Japan.;3. Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192 Japan.

Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Saitama, 332-0012 Japan.

Abstract:Point-of-care testing (POCT) devices have evolved to provide beneficial information about an individual's health whenever needed. Enzyme-based analytical devices have facilitated the highly selective detection of numerous biological molecules and ions. Enzymes are commonly used as the tags of recognition components, such as antibodies, to generate and amplify detection signals. Particularly, alkaline phosphatase (ALP) is one of the most widely used enzymes because of its high turnover number and low cost. Rapid response time and the incorporation of many sensors fabricated by micro/nano processing technologies are the advantages in using electrochemical devices as analytical tools. Therefore, ALP-based electrochemical devices have potential applications for more practical POCT platforms. This review summarizes recent research progress of ALP-based electrochemical devices for POCT. In addition to ALP substrates, the application of ALP-based immunosensors, aptasensors, and DNAzyme sensors are discussed.
Keywords:Analytical electrochemistry  Point-of-care testing  Alkaline phosphatase  Enzyme substrate
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号