Two new chiral EDTA-based metal chelates for weak alignment of proteins in solution |
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Authors: | Haberz Peter Rodriguez-Castañeda Fernando Junker Jochen Becker Stefan Leonov Andrei Griesinger Christian |
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Institution: | Max-Planck Institute for Biophysical Chemistry, Department of NMR-Based Structural Biology, Am Fassberg 11, 37077 G?ttingen, Germany. |
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Abstract: | structure: see text] A short synthesis of EDTA-based metal chelates that can be attached to the cysteine residue of a protein via a disulfide bond is described. The complexes were used after coordination of lanthanides to align trigger factor and apo-calmodulin in solution to yield residual dipolar couplings and pseudocontact shifts. Alignment tensors for the new tags are linearly independent compared to those of previously published tags. |
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