Electrochemical biosensors for determination of nystatin activity |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, PR China;2. Guangzhou Ingsens Sensor Technology Co. Ltd, Kaiyuan Road 11, Guangzhou 510535, PR China;1. Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China;2. Baoying Center for Control and Prevention of Aquatic Animal Infectious Disease, 30# Yeting East Road, Baoying 225800, China;3. Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China;4. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing 210008, China |
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| Abstract: | Nystatin is determined by recording the current of an oxygen-based biosensor or the potential of carbon dioxide-based biosensor. The yeast strain Saccharomyces cerevisiae is immobilized on an acetylcellulose filter and fixed to the surface of the electrochemical sensor. Both biosensors monitor the death of the strain the additiin of the antigungal solution and a measurable change of the curent (or potential), and the slope of the recording at the midpoint of the sigmoidal curve can be related to the nystatin activity. The carbondioxide-based biosensor provides a larger response range (100–500 U ml−1 nystatin) but the oxgen-based sensor is more sensitive (25–100 U ml−1 nystatin). All analyses are done at pH 4.5 where the response times of the electrochemical sensors are similar. |
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