比较蛋白质组学研究中的稳定同位素标记技术

比较蛋白质组学是指在蛋白质组学水平上研究正常和病理情况下细胞或组织中蛋白质表达变化,以期发现具有重要功能的生物标识物,为疾病的早期诊断提供依据。近年来它正成为蛋白质组学研究的热点和发展趋势。比较蛋白质组学的研究方法和策略有多种,本文就最近几年来稳定同位素标记技术(体内代谢标记技术和体外化学标记技术)在比较蛋白质组学研究中的进展进行综述。

Application of Stable Isotope Labeling in Comparative Proteomics

Comparative proteomics is the research of protein expression changing between normal and pathological cell or tissue on the proteome level. Potential biomarkers would be discovered from the research by comparative proteomics,which will be helpful to the diagnosis and therapy of diseases. In the recent years, it has been becoming the hot spot of the proteomics research and many strategies used in comparative proteomics have been developed. During those approaches, the strategies based on stable isotopic labeling coupled with mass spectrometry have been extensively used and lots of successful applications have been reported. In contrast to the traditional radioactive isotope labeling method, stable isotope labeling technique was not radioactive and the operation is simple. Metabolic labeling in vivo and chemical labeling in vitro are two parts of stable isotope labeling technique, which both have various advantages and disadvantages. This paper reviewed the progress of stable isotope labeling technique in comparative proteomics.